麻豆国产AV

Scholarship

Selected Recent Publications

• An integrated mechanism of G q regulation of PLC尾 enzymes, 2025
  Journal Article, Academic Journal
• Amylin receptor subunit interactions are modulated by agonists and determine signaling., 2025
  Journal Article, Academic Journal
• Visualization of endogenous G proteins on endosomes and other organelles, 2024
  Journal Article, Academic Journal
• In-Cell Arrestin-Receptor Interaction Assays., 2023
  Journal Article, Academic Journal
• GLP-1R signaling neighborhoods associate with the susceptibility to adverse drug reactions of incretin mimetics., 2023
  Journal Article, Academic Journal

Research Interests

My lab鈥檚 long-term objective is to understand how G protein-coupled receptors (GPCRs), heterotrimeric G proteins and other signaling proteins interact, and how these proteins are organized in specialized cells to produce physiological responses. Much of what we do requires the development of optical tools for imaging and spectroscopy in living cells, often taking advantage of rapid advances in structural biology, protein engineering and chemical biology. As specific examples, we engineered a resonance energy transfer (FRET and BRET) sensor to detect G protein activation (free Gbg subunits) in living cells, chemical and physical methods to immobilize and trap GPCRs in artificial microdomains on the cell surface, and a BRET method to monitor the subcellular trafficking of membrane proteins in real time. We have used these tools to gain insights into the process of receptor-G protein coupling and preassembly, G protein heterotrimer dissociation, the regulation of signaling kinetics by RGS proteins, and the quaternary structure of GPCRs. These methods have also been successfully adopted by many other labs.